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all trans retinoic acid atra  (Thermo Fisher)


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    Structured Review

    Thermo Fisher all trans retinoic acid atra
    All Trans Retinoic Acid Atra, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trans+retinoic+acid/all-trans-Retinoic+acid%2C+97%25/pmc12908063-58-50-44
    Average 95 stars, based on 1 article reviews
    all trans retinoic acid atra - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    FACS:

    Article Title: Vincristine treatment protects against podocyte damage in focal segmental glomerulosclerosis
    Article Snippet: .. Cells then underwent magnetic activated cell sorting to isolate podocytes (using biotin antipodoplanin antibody, BioLegend, 337015, conjugated to antibiotin microbeads, Miltenyi Biotec, 130-090-485) and glomerular endothelial cells (gECs, using anti-CD31 microbeads, Miltenyi Biotec, 130-091-935) before expansion in VRADD (RPMI-1640, ThermoFisher, 218750910 supplemented with 10% FBS, 1% penicillin/streptomycin, 100 nM 1,25(OH)2D3, 1 μM all trans retinoic acid and 100 nM dexamethasone) and gECs (Cell Biologics, H1168) media, respectively. .. We generated the GOAC in OrganoPlate 3-lane 40-well plates (Mimetas) as described.31,32 On consecutive days, a collagen solution (5 mg/ml AMSbio Cultrex 3D Collagen-I rat tail, 1M HEPES, 7 g/l NaHCO3) matrix was first generated in the middle channel before podocyte and then gEC seeding (20,000 of each/microchip).

    Recombinant:

    Article Title: A serum- and feeder-free system to generate CD4 and regulatory T cells from human iPSCs.
    Article Snippet: .. On day 50, the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid, 12.5 uL/mL of Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) and 10 ng/mL of CTS IL-2 (Thermofisher). ..

    Incubation:

    Article Title: The Influence of Lysosomal Stress on Dental Pulp Stem Cell-Derived Schwann Cells.
    Article Snippet: In brief, the DPSCs were grown for 24 h (h) in αMEM without FBS and supplemented with 1 mM of β-mercaptoethanol (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) on a Poly-Llysine-coated surface (Sigma-Aldrich, St. Louis, MO, USA). .. Hereafter, cells were incubated for 72 h with 35 ng/mL of trans-retinoic acid (Thermo Fisher Scientific, Waltham, MA, USA) in a standard αMEM supplemented with 5 μM of forskolin (Stemcell Technologies, Vancouver, BC, Canada), 10 ng/mL of basic fibroblast growth factor (Immunotools, Friesoythen, Biomolecules 2024, 14, 405 3 of 14 Germany), 5 ng/mL of platelet-derived growth factor AA (Immunotools, Friesoythen, Germany), and 200 ng/mL of Neuroregulin-1 (Immunotools, Friesoythen, Germany). ..

    Article Title: The Influence of Lysosomal Stress on Dental Pulp Stem Cell-Derived Schwann Cells
    Article Snippet: In brief, the DPSCs were grown for 24 h (h) in αMEM without FBS and supplemented with 1 mM of β-mercaptoethanol (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) on a Poly-L-lysine-coated surface (Sigma-Aldrich, St. Louis, MO, USA). .. Hereafter, cells were incubated for 72 h with 35 ng/mL of trans-retinoic acid (Thermo Fisher Scientific, Waltham, MA, USA) in a standard αMEM supplemented with 5 μM of forskolin (Stemcell Technologies, Vancouver, BC, Canada), 10 ng/mL of basic fibroblast growth factor (Immunotools, Friesoythen, Germany), 5 ng/mL of platelet-derived growth factor AA (Immunotools, Friesoythen, Germany), and 200 ng/mL of Neuroregulin-1 (Immunotools, Friesoythen, Germany). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Development of retinoid nuclear receptor pathway antagonists through targeting Aldehyde Dehydrogenase 1A3 (ALDH1A3)
    Article Snippet: .. All-trans retinoic acid was quantified as previously described.87 qPCR, western blot, and proliferation assay Quantitative real-time PCR (qRT-PCR) was performed using the SYBR green mastermix protocol on cDNA generated using the SuperScript IV cDNA assembly kit (Invitrogen) and the primers for human STRA6 (F 5’-GGGACAAGTTTCCGGGAGAG, R 5’-TCTGGCCCTTCTCCTCCAAT), human ALDH1A1 (F 5’- TGTTAGCTGATGCCGACTTG, R 5’-TTCTTAGCCCGCTCAACACT), human ALDH1A3 (F 5’-TCTCGACAAAGCCCTGAAGT, R 5’-TATTCGGCCAAAGCGTATTC), and human GAPDH (F 5’-GAAGGTGAAGGTCGGAGTC, R 5’-GAAGATGGTGATGGGATTTC). .. RNA was purified using the respective Qiagen kits.

    Western Blot:

    Article Title: Development of retinoid nuclear receptor pathway antagonists through targeting Aldehyde Dehydrogenase 1A3 (ALDH1A3)
    Article Snippet: .. All-trans retinoic acid was quantified as previously described.87 qPCR, western blot, and proliferation assay Quantitative real-time PCR (qRT-PCR) was performed using the SYBR green mastermix protocol on cDNA generated using the SuperScript IV cDNA assembly kit (Invitrogen) and the primers for human STRA6 (F 5’-GGGACAAGTTTCCGGGAGAG, R 5’-TCTGGCCCTTCTCCTCCAAT), human ALDH1A1 (F 5’- TGTTAGCTGATGCCGACTTG, R 5’-TTCTTAGCCCGCTCAACACT), human ALDH1A3 (F 5’-TCTCGACAAAGCCCTGAAGT, R 5’-TATTCGGCCAAAGCGTATTC), and human GAPDH (F 5’-GAAGGTGAAGGTCGGAGTC, R 5’-GAAGATGGTGATGGGATTTC). .. RNA was purified using the respective Qiagen kits.

    Proliferation Assay:

    Article Title: Development of retinoid nuclear receptor pathway antagonists through targeting Aldehyde Dehydrogenase 1A3 (ALDH1A3)
    Article Snippet: .. All-trans retinoic acid was quantified as previously described.87 qPCR, western blot, and proliferation assay Quantitative real-time PCR (qRT-PCR) was performed using the SYBR green mastermix protocol on cDNA generated using the SuperScript IV cDNA assembly kit (Invitrogen) and the primers for human STRA6 (F 5’-GGGACAAGTTTCCGGGAGAG, R 5’-TCTGGCCCTTCTCCTCCAAT), human ALDH1A1 (F 5’- TGTTAGCTGATGCCGACTTG, R 5’-TTCTTAGCCCGCTCAACACT), human ALDH1A3 (F 5’-TCTCGACAAAGCCCTGAAGT, R 5’-TATTCGGCCAAAGCGTATTC), and human GAPDH (F 5’-GAAGGTGAAGGTCGGAGTC, R 5’-GAAGATGGTGATGGGATTTC). .. RNA was purified using the respective Qiagen kits.

    SYBR Green Assay:

    Article Title: Development of retinoid nuclear receptor pathway antagonists through targeting Aldehyde Dehydrogenase 1A3 (ALDH1A3)
    Article Snippet: .. All-trans retinoic acid was quantified as previously described.87 qPCR, western blot, and proliferation assay Quantitative real-time PCR (qRT-PCR) was performed using the SYBR green mastermix protocol on cDNA generated using the SuperScript IV cDNA assembly kit (Invitrogen) and the primers for human STRA6 (F 5’-GGGACAAGTTTCCGGGAGAG, R 5’-TCTGGCCCTTCTCCTCCAAT), human ALDH1A1 (F 5’- TGTTAGCTGATGCCGACTTG, R 5’-TTCTTAGCCCGCTCAACACT), human ALDH1A3 (F 5’-TCTCGACAAAGCCCTGAAGT, R 5’-TATTCGGCCAAAGCGTATTC), and human GAPDH (F 5’-GAAGGTGAAGGTCGGAGTC, R 5’-GAAGATGGTGATGGGATTTC). .. RNA was purified using the respective Qiagen kits.

    Generated:

    Article Title: Development of retinoid nuclear receptor pathway antagonists through targeting Aldehyde Dehydrogenase 1A3 (ALDH1A3)
    Article Snippet: .. All-trans retinoic acid was quantified as previously described.87 qPCR, western blot, and proliferation assay Quantitative real-time PCR (qRT-PCR) was performed using the SYBR green mastermix protocol on cDNA generated using the SuperScript IV cDNA assembly kit (Invitrogen) and the primers for human STRA6 (F 5’-GGGACAAGTTTCCGGGAGAG, R 5’-TCTGGCCCTTCTCCTCCAAT), human ALDH1A1 (F 5’- TGTTAGCTGATGCCGACTTG, R 5’-TTCTTAGCCCGCTCAACACT), human ALDH1A3 (F 5’-TCTCGACAAAGCCCTGAAGT, R 5’-TATTCGGCCAAAGCGTATTC), and human GAPDH (F 5’-GAAGGTGAAGGTCGGAGTC, R 5’-GAAGATGGTGATGGGATTTC). .. RNA was purified using the respective Qiagen kits.



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